A near-infrared fluorescent probe for detecting hydrazine metabolized from isoniazid in living cells

Isoniazid is a drug for treating tuberculosis, but hydrazine (N2H4), the major metabolite of isoniazid, can cause hepatotoxicity. Therefore, monitoring the content of N2H4 in time is of great significance for studying the hepatotoxicity induced by isoniazid. In this study, a near-infrared fluorescent probe (BC-N) was designed and synthesized based on the specific reaction of acetyl ester with N2H4. BC-N exhibits excellent selectivity, sensitivity, and biocompatibility. In addition, BC-N is applied in the visualization of N2H4 produced from isoniazid in living cells and is a potential tool for monitoring hepatotoxicity induced by isoniazid. A near-infrared fluorescent probe (BC-N) was designed and synthesized based on the specific reaction between acetyl ester and N2H4, which can be applied to visualize N2H4 produced by isoniazid in living cells.image