Affinity-peptide-mediated multi-enzyme self-assembly system enhances dioxygenase catalyzing C–H hydroxylation via in situ α-ketoglutarate generation and H2O2 elimination

Huan Liu,Lunjie Wu, Jie Gu, Donglin Zhao, Shiyi Xin,Yan Xu,Yao Nie

Molecular Catalysis（2024）

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Abstract

•Affinity peptides successfully mediates the self-assembly of multiple enzymes.•Multi-enzyme complexes enable in situ elimination of H2O2 from the cascade system.•Multi-enzyme assembly facilitates Fe(II)/α-KG-dependent dioxygenase catalysis.•(2S,3R,4S)-4-hydroxyisoleucine was produced via one-pot cascade reaction.

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Key words

Multi-enzyme complex,Fe(II)/α-ketoglutarate-dependent dioxygenase,Hydroxyl amino acid biosynthesis,C-H hydroxylation,Biocatalysis,In situ cosubstrate generation

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