Adeno-Associated Virus Analysis by Size Exclusion Chromatography within 3 Minutes Using Short Bio-Inert Columns made with 3 µm Particles Operated at High Flowrates

•Current SEC analysis of AAVs mimic protein analysis and are suboptimal.•SEC of AAVs with columns of smaller dimensions save analysis time and sample.•Smaller columns can be operated at high flowrates without affecting results.•Adequate AAV sample handling is critical to successful quantitation of aggregates.