Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing.

Peter J Houweling,Vanessa Crossman,Chrystal F Tiong,Chantal A Coles,Rhonda L Taylor,Joshua S Clayton,Alison Graham,Katerina Vlahos,Sara E Howden,Kathryn N North

Stem cell research（2024）

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Abstract

We used gene editing to introduce DNA sequences encoding the tdTomato fluorescent protein into the α -skeletal actin 1 (ACTA1) locus to develop an ACTA1-tdTomato induced pluripotent stem cell reporter line for monitoring differentiation of skeletal muscle. This cell line will be used to better understand skeletal muscle maturation and development in vitro as well as provide a useful tool for drug screening and the evaluation of novel therapeutics for the treatment of skeletal muscle disease.

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