Development of a bacterial gene transcription activating strategy based on transcriptional activator positive feedback
Journal of Advanced Research(2023)
Abstract
•Transcription activation by positive regulators and their regulating sequences.•Positive regulator stimulates transcription of itself and downstream genes.•Higher transcription activation than strong constitutive promoters.•Demonstrated in Pseudomonas stutzeri, Escherichia coli and Streptomyces albus.•Highest yields of indole-3-acetic acid and prodigiosin in heterologous hosts.
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Key words
Transcription activation,Nitrogen fixation,Heterologous expression,Biosynthesis,Pathway engineering
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