A novel fluorescent probe based on phenothiazine for recognition of Ni2+and ClO- and its applications

In order to specifically detect Ni2+ and ClO-, a double functional s-triazine-phenothiazine fluorescent probe (BPTD) was synthesized, in which the multiple nitrogen atoms in s-triazine and hydrazone connecting arm were used for recognizing Ni2+, sulfur atom in phenothiazine was used as the reaction site for recognizing ClO-. BPTD has excellent optical properties: a large Stokes shift (131 nm) and good stability in a wide pH range (3.0012.00). Compared to the two fluorophores (s-triazine and phenothiazine), fluorescence emission peak of BPTD produces a large red-shift. The spectral experimental results show that BPTD can detect Ni2+ (5 - 80 mu M) and ClO- (10 - 40 mu M) with high selectivity and strong anti-interference. Compared with the reported fluorescent probes for detection of Ni2+, BPTD can detect Ni2+ more sensitively (response time 2.5 min, red shift 26 nm, the limit of detection (LOD) 98 nM), and simultaneously achieve the goal of highly sensitive detection of ClO- (response time 20 s, blue shift 18 nm, LOD 96 nM). BPTD can be applied to qualitative and quantitative detection of Ni2+ and ClO- in actual water samples, and made into portable test paper for rapid detection of Ni2+ and ClO-.