An arabinose-rich heteropolysaccharide isolated from Belamcanda chinensis (L.) DC treats liver cancer by targeting FAK and activating CD40

An undisclosed polysaccharide, BCP80-2, was isolated from Belamcanda chinensis (L.) DC. Structural investigation revealed that BCP80-2 consists of ten monosaccharide residues including t-alpha-Araf-(1-*, -*3,5)-alpha-Araf-(1-*, -*5)-alpha-Araf-(1-*, -*4)- -Xylp-(1-*, -*3)-alpha-Rhap-(1-*, -*4)- -Manp-(1-*, t- -Glcp-(1-*, -*6)-alpha-Glcp-(1-*, t- -Galp- (1-*, and-*3)-alpha-Galp-(1-*. In vivo activity assays showed that BCP80-2 significantly suppressed neoplasmic growth, metastasis, and angiogenesis in zebrafish. Mechanistic studies have shown that BCP80-2 inhibited cell migration of HepG2 cells by suppressing the FAK signaling pathway. Moreover, BCP80-2 also activated immunomodulation and upregulated the secretion of co -stimulatory molecules CD40, CD86, CD80, and MHC-II. In conclusion, BCP80-2 inhibited tumor progression by targeting the FAK signaling pathway and activating CD40-induced adaptive immunity.