Genotype-dependent N-glycosylation and newly exposed O-glycosylation affect plasmin-induced cleavage of histidine-rich glycoprotein (HRG)

Histidine-rich glycoprotein (HRG) is an abundant plasma protein harboring at least three N-glycosylation sites. HRG integrates many biological processes such as coagulation, antiangiogenic activity, immune complex clearance and pathogen clearance. Importantly, HRG is known to exhibit five genetic variants with minor allele frequencies of more than 10%, meaning these all exist with substantial frequencies in the human population. Among them, Pro204Ser can induce a fourth N-glycosylation site at Asn202.