A new phenylspirodrimane derivative from the deep-sea-derived fungus Stachybotrys chartarum FS705

A new phenylspirodrimane derivative named stachybotrysin A (1), together with four known analogues (2-5) were isolated and purified from the solid culture of the deep-sea-derived Stachybotrys chartarum FS705. Their structures were determined by comprehensive spectroscopic analysis and the absolute configuration was evaluated by theoretical ECD calculations. Compounds 1-5 were evaluated for their cytotoxic, antibacterial and a-glucosidase inhibitory activities. The results showed that compound 2 displayed mild cytotoxicity with IC50 values in the range of 8.88 -22.73 mu M against four human tumour cell lines, SF-268, MCF-7, HepG-2, and A549. Compound 1 showed strong a-glucosidase inhibitory activity with an IC50 value of 20.68 mu M. Compounds 4 and 5 exhibited weak antibacterial activity against Bacillus subtilis. [Graphical Abstract]