Immobilization of α-amylase onto functionalized molybdenum diselenide nanoflowers (MoSe2-NFs) as scaffolds: Characterization, kinetics, and potential applications in starch-based industries

•Hydrothermal synthesis was used to create MoSe2-NFs easily and sustainably.•The investigation focused on the covalent immobilization of α-amylase onto functionalized MoSe2-NFs.•The Box-Behnken design was utilized to optimize the parameters, leading to an immobilization efficiency of 87.33%.•Immobilization was confirmed by XRD, Raman, SEM, TEM, FT-IR, AFM, and Fluorescence microscopy.•Immobilization improves enzyme pH, temperature, and stability.