Design of Integrated System for Detection of Micro-organisms with Fabrication and Testing of ZnO Nanorods based Biosensor.

Efficient and reliable non-invasive methods for micro-organism detection have significant implications in various fields such as medical diagnostics, environmental monitoring, and food safety. Existing detection techniques, such as culturebased methods, polymerase chain reaction (PCR) have limitations including time-consuming processes, expensive equipment requirements and invasive sampling procedures. These drawbacks emphasize the need for alternate detection techniques that are non-invasive, rapid, sensitive, specific, and user-friendly. For this purpose, in this research, we present a portable integrated system for micro-organism detection, which is based on electrochemical impedance spectroscopy (EIS). The proposed system includes Zinc Oxide (ZnO) nanorods based interdigitized electrode (IDE) sensor and an EIS circuit. This work also presents the considerations and methodology for the fabrication of ZnO nanorods with high-yield. To validate the efficacy of the proposed methods, experimental results through scanning electron microscope are also presented. Finally, measurement results for detection of a micro-organism (yeast) is also presented to demonstrate the utility and effectiveness of the proposed methods and system.