Algerian propolis from distinct geographical locations: chemical profiles, antioxidant capacity, cytotoxicity and inhibition of 5-lipoxygenase product biosynthesis
CHEMISTRY & BIODIVERSITY(2024)
摘要
Propolis was collected from honeybee hives in three geographically distinct Algerian climates and extracts were characterized for composition and bioactivity. Bees were identified as native subspecies using an in-silico DraI mtDNA COI-COII test. Over 20 compounds were identified in extracts by LC-MS. Extracts from the Medea region were more enriched in phenolic content (302 +/- 28 mg GAE/g of dry extract) than those from Annaba and Ghardaia regions. Annaba extracts had the highest flavonoid content (1870 +/- 385 mg QCE/g of dry extract). Medea extracts presented the highest free-radical scavenging activity (IC50=13.5 mu g/mL) using the DPPH radical assay while Ghardaia extracts from the desert region were weak (IC50>100 mu g/mL). Antioxidant activities measured using AAPH oxidation of linoleic acid were similar in all extracts with IC50 values ranging from 2.9 to 4.9 mu g/mL. All extracts were cytotoxic (MTT assay) and proapoptotic (Annexin-V) against human leukemia cell lines in the low mu g/mL range, although the Annaba extract was less active against the Reh cell line. Extracts inhibited cellular 5-lipoxygenase product biosynthesis with IC50 values ranging from 0.6 to 3.2 mu g/mL. Overall, examined propolis extracts exhibited significant biological activity that warrant further characterization in cellular and in vivo models.
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关键词
Algerian honey bees,Anti-inflammatory,Antioxidants,Cytotoxicity,Propolis
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