Synthesized uniform-different sizes silver nanoparticles using TSC and SBH simultaneously for antibacterial application

Silver nanoparticles (AgNPs) in the form of nanospheres from a few nm to 100 nm in diameter were synthesized in a controlled manner using a combination of two reducing agents: sodium borohydride (SBH) and trisodium citrate (TSC). The influence of the size of AgNPs on antibacterial activity was investigated with different concentrations of AgNPs on two types of bacteria: Pseudomonas aeruginosa (PA) and Staphylococcus aureus resistant (SA) while the positive control was Ampicillin (Amp) 50 mu g/ml and the negative control was water. AgNPs were investigated for morphology, size and size distribution using transmission electron microscopy (TEM) and dynamic light scattering (DLS) measurements. The optical properties of the AgNPs were investigated by recording their UV-vis absorption spectra. The antimicrobial activity of AgNPs was determined using the disc diffusion method. The results showed that the antibacterial ability of AgNPs depends on both concentration and particle size. With a particle concentration of 50 mu g ml-1, the antibacterial ability is the best. The smaller the particle size, the higher the antibacterial ability. The simultaneous use of two reducing agents TSC and SBH is the novelty of the article to synthesize AgNPs particles that are uniform in shape and size while controlling the particle size. On that basis, their antibacterial performance is increased.