Characterization and low-temperature biodegradation mechanism of 17 -estradiol-degrading bacterial strain Rhodococcus sp. RCBS9

Steroidal estrogens residues in the environment can be a serious hazard to humans and animals and has been listed as group 1 carcinogens by World Health Organization (WHO). Microbial degradation is one of the effective strategies for the removal of such contaminants. In this study, a low-temperature degrading bacterial strain (Rhodococcus sp. RCBS9) was isolated from the soil of a dairy farm for 17 beta-estradiol (E2) degradation. The strain RCBS9 exhibited an efficient degradation potential at low temperatures. To lean how different factors influence E2 degradation, we have found a major role of intracellular enzymes in E2 degradation. Genomic and metab-olomic analyses have suggested potential degradation genes and four metabolic pathways. These findings pro-vide valuable strain resources for the low temperature bioremediation of E2 contamination and insights into E2 biodegradation mechanism.