A High-Resolution Prediction Network for Predicting Intratumoral Distribution of Nanoprobes by Tumor Vascular and Nuclear Feature

In this study, the critical need for precise and accurate prediction of intra-tumor heterogeneity related to the enhanced permeability and retention effect and spatial distribution of nanoprobes is addressed for the development of effective nanodrug delivery strategies. Current predictive models are limited in terms of resolution and accuracy, prompting the construction of a high-resolution prediction network (HRPN) that estimates the microdistribution of quantum dots, factoring in tumor vascular and nuclear features. The HRPN algorithm is trained using 27 780 patches and validated on 4920 patches derived from 4T1 breast cancer whole-slide images, demonstrating its reliability. The HRPN model exhibits minimal error (mean square error = 1.434, root mean square error = 1.198), satisfactory goodness of fit (R2 = 0.891), and superior image quality (peak signal-to-noise ratio = 44.548) when compared to a generative-adversarial-network-structured model. Furthermore, the HRPN model offers improved prediction accuracy, broader prediction intervals, and reduced computational resource requirements. Consequently, the proposed model yields high-resolution predictions that more closely resemble actual tumor microdistributions, potentially serving as a powerful analytical tool for investigating the spatial relationship between the tumor microenvironment and nanoprobes. Herein, a high-resolution prediction network (HRPN), which predicts the microdistribution of quantum dots, is built utilizing tumor vascular and nuclear features. HRPN automatically learns 27 780 patches and is tested in 4920 patches, decomposed from 4T1 breast cancer whole-slide images, proving the reliability.image (c) 2023 WILEY-VCH GmbH