Dihydromyricetin improves rhythm by anti-oxidative stress damage in sleep deprivation Drosophila

Consistently sleeping 5 hours or less per night will increase the development of various health issues. Dihydromyricetin (DHM) could reduce oxidative stress and promote longevity, but its effectiveness in alleviating sleep deprivation remains unclear. Thus, we established a sleep-deprived Drosophila model by prolonged illumination, named light-prolonged Drosophila (LPD), to explore this problem. Results showed that DHM enhanced the sleep quality of the LPD and expanded their lifespan, by reducing their nocturnal activities, decreasing intestinal epithelial cell mortality, and restoring glucose and glutathione levels. Transcriptome analysis and RT-qPCR results showed that DHM has the potential to modulate Drosophila's gut immunity and mitigate mitochondrial oxidative stress. Furthermore, we found that DHM can inhibit the expression of TIM, Pi3k, and TOR, then decrease Tsc1, while simultaneously promoting FoxO and Thor. In conclusion, DHM could be used as an effective intervention for enhancing sleep quality and counteracting the detrimental of sleep deprivation on lifespan.