Usefulness and analytical performances of complement multiplex assay for measuring complement biomarkers in plasma

•The complement system is implicated in 50+ diseases with varied mechanisms of activation of deficits.•Accurately assessing the level of complement activation remains a challenge.•Quantifying complement activation fragments and protein could provide an instantaneous snapshot of the cascade's activation state.•We presented the use of complement multiplex ELISA for simultaneously measuring 14 complement proteins.•The data presented here pave the way for the utilization of the multiplex ELISA technique for quantifying complement proteins and fragments.