Schizophyllan from Schizophyllum commune BRM 060008: Potential application as an inhibitor of lipase
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES(2024)
摘要
beta-D-glucan has significant implications in regulating lipid metabolism and preventing diseases associated with lipid accumulation. Schizophyllan (SPG) from Schizophyllum commune fungus is a commercially important beta-glucan with applications in the health food industry, pharmacy, and cosmetics. However, SPG was obtained by submerged culture of the wood-rotting and filamentous fungus S. commune BRM 060008, which may have been isolated from the Cerrado Biome of Brazil. In this study, to confirm that the polysaccharide produced by BRM 060008 strain fermentation was indeed (1 -> 3)(1 -> 6)-beta-D-glucan, it was purified and characterized using Fourier transform infrared spectroscopy, thermogravimetric analysis, high-performance size exclusion chromatography, nuclear magnetic resonance, and methylation analysis. The polysaccharide produced was identified as the beta-Dglucan expected with a high molecular weight (1.093 x 10(6) g/mol) and the thermogravimetric analysis indicated a maximum degradation temperature of similar to 324 degrees C and a 60 % residual weight, lower than commercial SPG. The molecular structure and thermal properties of the beta-D-glucan were similar to the commercial sample. Additionally, the in vitro pancreatic lipase inhibitory activity was evaluated, investigating anti-obesity and antilipidemic properties. The results showed unprecedented lipase inhibition activity to SPG prepared using the S. commune strain BRM 060008, making it promising for food and pharmaceutical applications.
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关键词
Schizophyllan,Schizophyllum commune,beta-(1 -> 3)(1 -> 6)-Glucan,Brazilian native strain,In vitro lipase inhibitory activity
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