Riparian vegetation plastic monitoring: A harmonized protocol for sampling macrolitter in vegetated riverine habitats

Many studies highlighted that rivers transported land -based plastics to the sea. However, most of the litter remains stuck in the fluvial ecosystem, also blocked by vegetation. To date, research on riverine macrolitter focused on floating and riverbank monitoring, thus methods to sample riverbank and floating litter have been developed. Concerning rivers, few recent studies highlighted the role of riparian vegetation in entrapping plastics. Given that vegetation represents a large part of riverine ecosystems and that the dynamics of plastics entrapped by vegetation are neglected, it appears pivotal to study in more detail how vegetation contributes to plastic retention. However, as current protocols and guidelines considered only floating and riverbank plastics without providing standardized and updated strategies to monitor litter in vegetation, here we aimed to develop a new standardized protocol and tools to assess plastics in vegetation. Specifically, we focused on unveiling the three -tridimensional structure of vegetation in relation to plastic occurrence, while considering seasonal and hydromorphological aspects. To investigate the trapping effect of vegetation, we developed a three-dimensional vegetation structure index (3DVI) related to plastics. The 3DVI index considers plant structure (i.e., number of branches) and diversity (i.e., species). To test the 3DVI, we conducted an in -situ case study in central Italy. We found that both primary and secondary riparian vegetation blocked plastic litter. In detail, 3DVI correlated with the number of plastics, highlighting that the densest and most diverse communities trap more plastics. Furthermore, we provided for the first time the assessment of seasonality for the macroplastic entrapment by riparian vegetation and a preliminary quantification of wind-blown plastics. Our results should be of interest to promote the development of standardized and harmonized monitoring strategies for riparian habitat management and conservation.