转Pofst3糙皮侧耳菌株农艺性状和胞外酶活性分析
Acta Edulis Fungi（2023）
Abstract
通过袋栽实验比较野生型、Pofst3过表达型和Pofst3反义沉默型糙皮侧耳(Pleurotus ostreatus)菌株(分别记为Ⅰ、Ⅱ、Ⅲ)的菌丝生长速度、原基数量、子实体数量、菌柄直径、菌盖直径等农艺性状;测定其漆酶、木聚糖酶、纤维素酶活性.结果表明:Ⅰ、Ⅱ、Ⅲ菌丝生长速度差异不显著;与Ⅰ相比,Ⅲ的原基和子实体数量多,菌柄直径大,菌盖直径小,原基和子实体中Pofst3的相对表达量低;与Ⅰ、Ⅱ相比,Ⅲ的漆酶、木聚糖酶及纤维素酶活性较高;Pofst3对糙皮侧耳发育的影响可能与其胞外酶活性相关.
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Key words
Pleurotus ostreatus,wild type,overexpression type,antisense silencing type,laccase,xylanase,cellulase
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