ITGB2-ICAM1 axis promotes liver metastasis in BAP1-mutated uveal melanoma with retained hypoxia and ECM signatures
Cellular Oncology(2023)
Abstract
Purpose Uveal melanoma (UM) with BAP1 inactivating mutations has a high risk of metastasis, but the mechanism behind BAP1 deficiency driving UM metastasis is unknown. Methods We analyzed the single-cell RNA sequencing (scRNA-Seq) data comprised primary and metastatic UM with or without BAP1 mutations (MUTs) to reveal inter- and intra-tumor heterogeneity among different groups. Then, an immune-competent mouse liver metastatic model was used to explore the role of ITGB2-ICAM1 in BAP1-associated UM metastasis. Results Cluster 1 tumor cells expressed high levels of genes linked to tumor metastasis, such as GDF15 , ATF3 , and CDKN1A , all of which are associated with poor prognosis. The strength of communication between terminally exhausted CD8 + T cells and GDF15 hi ATF3 hi CDKN1A hi tumor cells was enhanced in BAP1-mutated UM, with CellChat analysis predicting strong ITGB2-ICAM1 signaling between them. High expression of either ITGB2 or ICAM1 was a worse prognostic indicator. Using an immune-competent mouse liver metastatic model, we indicated that inhibiting either ICAM1 or ITGB2 prevented liver metastasis in the BAP1-mutated group in vivo. The inhibitors primarily inhibited hypoxia- and ECM-related pathways indicated by changes in the expression of genes such as ADAM8 , CAV2 , ENO1 , PGK1 , LOXL2 , ITGA5 , and VCAN . etc. Conclusion This study suggested that the ITGB2-ICAM1 axis may play a crucial role for BAP1-associated UM metastasis by preserving hypoxia- and ECM- related signatures, which provide a potential strategy for preventing UM metastasis in patients with BAP1 mutation.
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Key words
Uveal melanoma,BAP1 mutation,Terminally exhausted CD8+ T,ITGB2,ICAM1
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