Highly stable glucose oxidase polynanogel@MXene/chitosan electrochemical biosensor based on a multi-stable interface structure for glucose detection

It is a challenging and meaningful task to design an enzyme electrochemical biosensor that can maintain high sensitivity while improving stability. In this study, we constructed an enzyme electrochemical biosensor by preparing nanocomposites with multi-stable interface structures. Specifically, the nanocomposite (PGOx@MXene/CS) was prepared by efficient electrostatic assembly of GOx polynanogel (PGOx) onto MXene nanosheets. PGOx could enhance enzyme stability, while the extensive the large specific surface area of MXene could realize the efficient loading of nanocapsules (PGOx) and catalyze the decomposition of toxic intermediate H2O2, thereby reducing its influence on the stability of enzyme. The linear range of the constructed glucose sensor was 0.03–16.5 mM, the sensitivity was 48.98 μA mM−1·cm−2, and the detection limit was 3.1 μM. After 200 cycles, the current still remained at 85.83% of the initial current value. The high sensitivity, excellent selectivity and great reproducibility verified the effectiveness of the system we constructed. The multi-stable enzyme electrochemical biosensor had a wide application prospect in stable and continuous blood glucose detection.