A Comparative Analysis of the Properties of Recombinant Endoinulinase, Exoinulinase, Sucrase, and Alpha-Galactosidase C

O. A. Sinitsyna, E. A. Rubtsova, D. O. Osipov, E. G. Kondratieva, M. V. Semenova, A. I. Korolev, E. V. Yaroshenko, A. M. Rozhkova, V. A. Nemashkalov, A. P. Sinitsyn

Applied Biochemistry and Microbiology（2023）

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摘要

As a result of cloning of the inuA, inu1, aglC, and fopA genes encoding endoinulinase (endoINU), exoinulinase (exoINU), α-galactosidase C (AGLС) and sucrase (SUC), respectively, into the recipient strain Penicillium verruculosum B1-537 (ΔniaD), recombinant producer strains were obtained that are capable of producing target recombinant enzymes with a high yield (32‒50

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endoinulinase,exoinulinase,α-galactosidase,sucrase,inulin,sucrose,galactooligosaccharides,Penicillium verruculosum

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