An improved protocol for bacteria identification by MALDI-TOF MS directly from positive blood cultures
European Journal of Clinical Microbiology & Infectious Diseases(2023)
摘要
FASTinov® developed a rapid antimicrobial susceptibility test that includes the purification of a bacterial suspension directly from positive blood cultures (BC). In order to streamline laboratory workflow, the use of the bacterial suspension obtained through FASTinov® sample prep was tested for identification (ID) by matrix absorption laser deionization–time of flight mass spectrometry (MALDI-TOF MS) (Bruker) in 364 positive BC, and its accuracy assessed comparing with the MALDI-TOF MS ID of the next-day subcultured colonies. FASTinov sample prep was highly reliable for rapid ID directly from BC with proportion of agreement of 94.9
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关键词
MALDI-TOF,Mass spectrometry,Blood cultures,Rapid identification
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