Antidiabetic property of fractions and pure compounds from Andrographis paniculata

Chlorophyll-free fractions of Andrographis paniculata were investigated for glucose uptake and lipid reduction in 3T3-L1 adipocytes. At 25 mu g/ml, the acid fraction concentration enhanced glucose uptake by 82%. Basic and neutral fractions at 100 mu g/ml enhanced glucose uptake by 82% and 78%, respectively. The three fractions showed improved glucose uptake compared to the crude extract (25% uptake at 50 mu g/ml). GC-MS analysis of the fractions revealed the presence of chemicals with antidiabetic activities. The neutral fraction was prioritised for pure compound isolation to provide known andrographolide (1), 14-deoxyandrographolide (2), and a novel compound, 3-epi-11,12-didehydro-14-deoxyandrographolide (5). At a concentration of 1 mu M, compounds 2 and 5 are as effective as 10 mM metformin in glucose uptake. They also reduce lipid accumulations in 3T3-L1 adipocytes by decreasing the size and number of lipid droplets. The activities of fractions and compounds support the use of A. paniculata in treating type 2 diabetes. [GRAPHICS]