Reverse Genetics of Hepatitis C Virus Using an RNA Polymerase I-Mediated Transcription.
Methods in molecular biology (Clifton, N.J.)(2024)
摘要
The reverse genetics system commonly used for the production of hepatitis C virus (HCV), which is a major causative agent of liver diseases, involves introduction of the viral genomic RNA synthesized in vitro into human hepatoma cells by electroporation. As an alternative methodology, we describe a cell culture system based on transfection with an expression plasmid containing a full-length HCV cDNA clone flanked by RNA polymerase I promoter and terminator sequences to generate infectious virus particles from transfected cells.
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