Vascular Response to Low-intensity Ultrasound Stimulation.

Low-intensity ultrasound stimulation (LIUS) is an emerging neuro- and vascular-modulation technique. Studies on humans and animals have shown that LIUS could induce changes in neuronal oscillations or blood flow. However, it is still inconclusive whether the hemodynamic response to LIUS is due to neurovascular coupling (NVC), direct ultrasound-vessel interactions, or both. This study aims to detect the direct effect of LIUS on vessels using optical imaging. Fluorescence images with indocyanine green (ICG) were used to identify and quantify the morphological change in the auricle vessels of rats. Diameters of vessels were measured before, during, and post LIUS. The results indicated that LIUS could significantly increase the vessel diameters (p = 0.031). Further exploratory analysis showed that vessel dilation occurred among the majority of randomly selected vessels (i.e., 21/30 animals (70%), dilation: 6.84±1.95µm, 95% CI: [3.02,10.66]), with a significant confounding effect of the vessel size. The results provided indirect evidence for two distinct pathways in LIUS-based neurovascular modulation, i.e., the NVC and the direct ultrasound-vessel interactions.