Germination biology of susceptible and target-site (Ile-1781-Thr) herbicide resistant short-spiked canary grass (Phalaris brachystachys) subpopulations

Quantifying the level of seed germiabiliy of herbicide-resistant (R) and susceptible (S) weeds is useful for understanding the evolutionary development of herbicide resistance, but also for implementing herbicide-resistance management strategies. Germination is a crucial aspect in the life phase of weeds. Phalaris brachystachys biotypes resistant to ACCase-inhibiting herbicides have been confirmed in wheat fields in Iran. This study aimed to investigate the germination behaviour of ACCase- resistant and susceptible subpopulations P. brachystachys under different environmental factors. An analysis of the seed germination of P. brachystachys sub populations at the seed stage was therefore conducted. The resistant (R) and susceptible (S) P. brachystachys subpopulation germination traits were tested in different temperature, salinity stress, drought, and burial depth conditions. All tests were carried out with five replications in a completely randomized design. The highest germination percentage in both subpopulations occurred at a temperature range of 20 to 25 °C. At 35 °C, no germination occurred. In terms of estimated cardinal temperatures, no differences were observed between S and R subpopulations. Seeds containing the ACCase Ile-1781-Thr mutation germinated better under salt and osmotic stress. There were no fitness differences in pH conditions. The percentage and rate of emergence of the R subpopulation were more than S subpopulation at different burial depths. The seedling emergence reached a maximum of 50