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Non-specific signals in real-time RT-PCR for detecting respiratory viruses

Gannon C. K. Mak, Derek L. L. Hung

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE（2024）

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Abstract
We describe our experiences in investigating the origin of non-specific signals during the development phase of a multiplex PCR assay for respiratory viruses. After ruling out various sources of error, eventually we discovered the non-specific signal was related to the particular lot of the PCR kit.
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Key words
PCR,Non-specific signals,Reagent lot
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