Brevibacillus ruminantium sp. nov., isolated from cow faeces

An aerobic, Gram- stain-positive, rod-shaped, endospore-forming bacterial strain, designated BB3- R-1T , was isolated from cow faeces sampled in Daejeon, Republic of Korea. Growth was observed at 25-45 degrees C (optimum, 35-40 degrees C) and pH 7.0-9.0 (optimum, pH 8.0), with up to 3 % (w/v) NaCl (optimum, 0 % NaCl). BLAST analysis of 16S rRNA gene sequences revealed the highest sequence similarity of strain BB3- R1(T) to Brevibacillus borstelensis NRRL NRS- 818(T) (98.8 %) followed by Brevibacillus panacihumi JCM 15085(T) (97.5 %). According to 16S rRNA gene and whole-genome based phylogenetic trees, strain BB3- R1(T) clustered with Brevibacillus composti FJAT-54423(T) and B. borstelensis NRRL NRS- 818(T). OrthoANI and dDDH values of strain BB3- R1(T) with the closely related strains were lower than 77.5 and 26.8 %, respectively. The major menaquinones and polar lipids of the strain were MK -7 and phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, respectively. The major fatty acids (>10 %) were C-14: 0 iso, C-15: 0 iso, C-15: 0 anteiso and C-16: 1 omega 7c alcohol. The cell -wall peptidoglycan contained cross-linked meso- diaminopimelic acid (type A1 gamma). The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that the strain represents a novel species of the genus Brevibacillus , for which the name Brevibacillus ruminantium sp. nov. (type strain BB3- R1(T)=KACC 22663(T)=NBRC 115962(T)) is proposed.