Simple quantitative method using HPLC-CAD for assessing plasmalogen vinyl ether phospholipids, applicable to food

In recent years, considering throughput, a simple quantitative method using normal -phase liquid chromatography (NP-HPLC) has been established for the assessment of foods with functional claims that contain plasmalogens. To certify the content of functionally related ingredients, we aimed to verify the validity of the analytical values obtained with this method. We built a simple quantitative method using NP-HPLC equipped with charged aerosol detector (NP-HPLC simple method) to quantify phosphatidylethanolamine, phosphatidylcholine, and their subclasses in a soft capsule product which is classified as a food with functional claims. The amount of plasmalogens per 2 soft capsules, which is the recommended daily intake, was determined and compared with value obtained using the 2D-HPLC method. As a result, the validity of the analytical value was confirmed. We believe that the NP-HPLC simple method can be used to assess plasmalogens in not only soft capsules but also other foods.