First Report of Colletotrichum siamense Causing Leaf Spot on Jasminum sambac in China

HomePlant DiseaseVol. 107, No. 7First Report of Colletotrichum siamense Causing Leaf Spot on Jasminum sambac in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Colletotrichum siamense Causing Leaf Spot on Jasminum sambac in ChinaMingyan Sun, Chunniu Li, Xianmin Li, Zhaoyang Bu, Yanfei La, Kedan Liu, and Qili LiMingyan SunFlowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, ChinaSearch for more papers by this author, Chunniu LiFlowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, ChinaSearch for more papers by this author, Xianmin LiFlowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, ChinaSearch for more papers by this author, Zhaoyang Bu†Corresponding author: Z. Bu; E-mail Address: [email protected]https://orcid.org/0000-0002-7605-7228Flowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, ChinaSearch for more papers by this author, Yanfei LaFlowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, ChinaSearch for more papers by this author, Kedan LiuFlowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, ChinaSearch for more papers by this author, and Qili Lihttps://orcid.org/0000-0001-5775-8910Institute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi 530007, ChinaSearch for more papers by this authorAffiliationsAuthors and Affiliations Mingyan Sun1 Chunniu Li1 Xianmin Li1 Zhaoyang Bu1 † Yanfei La1 Kedan Liu1 Qili Li2 1Flowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China 2Institute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi 530007, China Published Online:1 Jul 2023https://doi.org/10.1094/PDIS-07-22-1547-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleJasmine (Jasminum sambac (L.) Aiton) is cultivated as a commercial floricultural crop in many countries around the world (Gao et al. 2020). From June to August 2020, leaf spots on jasmine were observed on a jasmine plantation in Hengzhou, Guangxi province. Over 40% of the plants in 6 ha fields were infected. This disease was prevalent in jasmine production areas of China (Chen et al. 2012; Du et al. 2020). Symptoms began as chlorotic regions (from 5 to 10 mm in diameter) with light brown necrotic centers, which gradually expanded to the entire leaf. Eventually, the disease led to defoliation and dieback. The edges of affected parts from diseased leaves were cut into pieces (3 mm2). Pieces were treated with 75% ethanol for 10 s, soaked in 2% NaClO solution for 1 min, washed three times with sterile water, and incubated on potato dextrose agar (PDA) plates at 28°C for 5 days in the dark. Fungal cultures that showed similar morphological characteristics were isolated, and three representative isolates (HL6-1 to HL6-3) were purified following Mo et al. (2018). The cultures on PDA changed from white to dark gray after 7 days and produced conidiomata after 14 days. Conidia were hyaline, one celled, guttulate, cylindrical, and 12.07 to 18.09 × 4.04 to 8.05 μm, 13.17 to 16.35 × 4.22 to 6.13 μm, and 10.11 to 22.17 × 3.65 to 8.1 μm for HL6-1, HL6-2, and HL6-3, respectively. Gray-brown or dark brown appressoria formed from conidia were subglobose or elliptical. Conidial appressoria and mycelial appressoria were 5.53 to 13.96 × 3.58 to 13.95 μm and 4.24 to 14.01 × 2.4 to 10.86 μm. Genomic DNA was extracted from three isolates and the partial internal transcribed spacer (ITS) regions, intergenic region of apn2 and MAT1-2-1 (ApMAT), and fragments of actin (ACT), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), chitin synthase (CHS-1), and β-tubulin (TUB2) genes were amplified, sequenced, and submitted to GenBank (ITS, ON115173 to ON115175; ApMat, ON156517 to ON156519; ACT, ON146469 to ON146471; GAPDH, ON156502 to ON156504; CHS-1, ON156507 to ON156509; TUB-2, ON156512 to ON156514). A phylogenetic tree was constructed with MrBayes v. 3.2.6 and MEGA v. 10.1.5 based on the concatenation of multiple sequences. The three isolates were grouped with strain Colletotrichum siamense ICMP 18578. Results indicated the three isolates were identified as C. siamense Prihastuti, L. Cai & K.D. Hyde. To confirm the pathogenicity of the three isolates, four sets (five plants per set) of 160 healthy leaves of 2-year-old plants (J. sambac, eight leaves per plant) were slightly scratched with a sterilized toothpick at each of eight locations. Conidial suspensions (1 × 106 conidia/ml) in 0.1% Tween 20 were inoculated onto each wounded spot of three sets as the treatment groups, and wounded leaves treated with sterile water were the control. All plants were covered with plastic bags and cultivated in a phytotron (12 h/12 h light/dark, 28°C). After 7 days, irregular chlorotic regions with brown lesions were observed on inoculated leaves while no symptoms appeared on controls. The same fungi were reisolated from inoculated leaves and confirmed by morphological and molecular identification, fulfilling Koch’s postulates. C. siamense has been associated with leaf anthracnose of J. sambac in Vietnam (Wikee et al. 2011) and Jasminum mesnyi in China (Zhang et al. 2019). To our knowledge, this is the first report of C. siamense causing jasmine anthracnose in China, which provides a reference for the management of this disease.The author(s) declare no conflict of interest.References:Chen, Y. S., et al. 2012. Tea in Fujian 5:31. Google ScholarDu, C. J., et al. 2020. J. Anhui Agric. Sci 48:168. Google ScholarGao, W., et al. 2020. Plant Dis. 105:501. https://doi.org/10.1094/PDIS-06-20-1222-PDN Link, ISI, Google ScholarMo, J. Y., et al. 2018. Plant Dis. 102:1283. https://doi.org/10.1094/pdis-09-17-1516-re Link, ISI, Google ScholarWikee, S., et al. 2011. Fungal Divers. 46:171. https://doi.org/10.1007/s13225-010-0049-x Crossref, ISI, Google ScholarZhang, Y. L., et al. 2019. Plant Dis. 103:2675. https://doi.org/10.1094/pdis-04-19-0804-pdn Link, ISI, Google ScholarFunding: The research was supported by Guangxi Science and Technology Program (GUIKEAB18221064), Science and Technology Major Project of Guangxi (GUIKEAA17204046-1), and Foundation for Development of Science and Technology Guangxi Academy of Agricultural Sciences (GNK2021JM112, GNK2021YT134, GNK2021JM29).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 107, No. 7 July 2023SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 26 Jul 2023Published: 1 Jul 2023First Look: 5 Dec 2022Accepted: 29 Nov 2022 Page: 2225 Information© 2023 The American Phytopathological SocietyFundingGuangxi Science and Technology ProgramGrant/Award Number: GUIKEAB18221064Science and Technology Major Project of GuangxiGrant/Award Number: GUIKEAA17204046-1Foundation for Development of Science and Technology Guangxi Academy of Agricultural SciencesGrant/Award Number: GNK2021JM112Grant/Award Number: GNK2021YT134Grant/Award Number: GNK2021JM29KeywordsColletotrichum siamenseJasminum sambacleaf spotThe author(s) declare no conflict of interest.PDF download