Appropriate Condition for Cluster Buds Induction of Cremastra appendiculata

To screen for suitable conditions for cluster bud induction of Cremastra appendiculata . The aseptic germination protocorms of C. appendiculata seeds were used as explant materials to explore the effects of different basic media, types of plant growth regulators, anti-browning agents and their concentrations on the induction of cluster buds of C. appendiculata . The results showed that: 1/4MS medium was the suitable basic medium for cluster buds growing; when the concentration of plant growth regulator TDZ was 2.0 mg/L and the concentration of IAA was 0.2 mg/L, the induction rates of cluster bud were higher which are 72.58% and 55.63% respectively; when the anti-browning agent was PVP and its concentration is 2 000 mg/L, the induction rate of cluster buds was higher which is 79.90%. The suitable conditions for C. appendiculata cluster buds induction are: 1/4MS + TDZ 2.0 mg/L+ IAA 0.1 mg/L+ PVP 2 000 mg/L, and the induction rate can reach 81.16%. This provided a basis for establishing a rapid propagation technology system of C. appendiculata and realizing its large-scale artificial cultivation.