Pomegranate (Molasses, White Peel, Red Peel) effect in reducing Phenylhydrazine-induced Spleen injury and hemolytic anemia incidence

Abstract In this study, the effects of pomegranate molasses, white peel extract, and red peel extract on male rats with phenylhydrazine -induced anaemia are discussed. Reduced body weight, haemoglobin concentration, hematocrit, red cell count, and red blood cell survival were the results of phenylhydrazine-induced anaemia. Adult haemoglobin type A, MPV, basophils, PDW, normal adult haemoglobin type A2, eosinophils, PDW, and PCT. Initial hemolysis due to fragility, complete hemolysis due to osmotic fragility, Adult haemoglobin type A, normal adult hemoglobin , MCHC, RDW-CV, PCT, and PDW, as well as eosinophils. In the histopathology analysis of the spleen, the PHZ exposed group had a higher incidence of macrophages carrying cellular debris as a result of degeneration inside the white pulp and red pulp with edematous gaps. Additionally, PHZ provided a magnified view of the final field that demonstrated the presence of macrophages inside the white pulp along with vacuolization and necrotic regions. A magnified view of the last field inside the red pulp is also shown, revealing a significant amount of necrotic cells, bleeding, and sporadic apoptotic cells. There was an increase Body Weight, G6PD, Hemoglobin Concentration, Heamatocrit, Red Cell Count, Red Blood Cell Survival, PDW, Normal Adult Hemoglobin Hb A2, MPV, MCHC, Basophils, Hemoglobin Adult Hb A, RDW, PCT, Eosinophils. The splenic parenchyma in the red and white pulps of PHZ-exposed rats receiving molasses pomegranate treatment showed a lesser degree of recovery and the presence of apoptotic cells along with bleeding and vacuolization. Strong antioxidants in molasses, white peel, and red peel of pomegranates preserve haematological and spleen tissue.