First Report of Nigrospora aurantiaca Causing Leaf Blight Disease of Peanut in China

HomePlant DiseaseVol. 107, No. 7First Report of Nigrospora aurantiaca Causing Leaf Blight Disease of Peanut in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Nigrospora aurantiaca Causing Leaf Blight Disease of Peanut in ChinaKang He, Xinying Song, Ying Li, Manlin Xu, Zhiqing Guo, Jing Yu, Xiaoyuan Chi, Xia Zhang, and Yucheng ChiKang HeShandong Peanut Research Institute, Qingdao, Shandong, ChinaSearch for more papers by this author, Xinying SongShandong Peanut Research Institute, Qingdao, Shandong, ChinaSearch for more papers by this author, Ying LiShandong Peanut Research Institute, Qingdao, Shandong, ChinaSearch for more papers by this author, Manlin Xuhttps://orcid.org/0000-0002-4266-5110Shandong Peanut Research Institute, Qingdao, Shandong, ChinaSearch for more papers by this author, Zhiqing GuoShandong Peanut Research Institute, Qingdao, Shandong, ChinaSearch for more papers by this author, Jing YuShandong Peanut Research Institute, Qingdao, Shandong, ChinaSearch for more papers by this author, Xiaoyuan ChiShandong Peanut Research Institute, Qingdao, Shandong, ChinaSearch for more papers by this author, Xia Zhang†Corresponding authors: X. Zhang; E-mail Address: [email protected], and Y. Chi; E-mail Address: [email protected]https://orcid.org/0000-0002-3631-4082Shandong Peanut Research Institute, Qingdao, Shandong, ChinaSearch for more papers by this author, and Yucheng Chi†Corresponding authors: X. Zhang; E-mail Address: [email protected], and Y. Chi; E-mail Address: [email protected]https://orcid.org/0000-0002-7157-3477Shandong Peanut Research Institute, Qingdao, Shandong, ChinaSearch for more papers by this authorAffiliationsAuthors and Affiliations Kang He Xinying Song Ying Li Manlin Xu Zhiqing Guo Jing Yu Xiaoyuan Chi Xia Zhang † Yucheng Chi † Shandong Peanut Research Institute, Qingdao, Shandong, China Published Online:16 Jun 2023https://doi.org/10.1094/PDIS-05-22-1134-PDNAboutSectionsPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In June 2021, a previously unreported leaf blight disease of peanut (Arachis hypogaea) was observed on field-grown peanut (Jinhua19) in Laixi city, Shandong province, China. Approximately 5% of plants showed disease symptoms in the fields we investigated. The symptoms first appeared as yellow round or irregular spots on leaves, and then the spots became brown. As the disease progressed, spots became larger and even converged, which later produced leaf chlorosis and abscission. Symptomatic leaves were cut into small pieces, surface disinfested with 70% ethanol for 30 s and 1% NaClO for 60 s, rinsed three times in sterile water, dried on sterile filter paper, placed on potato dextrose agar (PDA) media, and incubated at 25°C in darkness. Fungal cultures were initially white, with red pigment, then turned gray, and eventually turned black, and aerial hyphae were dense. Conidia were spherical or slightly ellipsoidal, black, smooth, and 8.6 to 11.5 × 8.7 to 14.5 μm (n = 50). Morphological characteristics of the isolates matched the description of Nigrospora aurantiaca (Wang et al. 2017). Molecular identification was performed by sequencing beta tubulin gene (TUB) with Bt2a/Bt2b and translation elongation factor 1-alpha (TEF) with EF1-728F/EF1-986R (Wang et al. 2022) of a representative isolate ZHX11. TUB (OK489789) and TEF (OK489790) of ZHX11 obtained 100% (401/401 nucleotides) and 99.64% (279/279 nucleotides) similarity to those of N. aurantiaca (MN329935, MN264010), respectively. Alignment was conducted separately for each gene set using Clustal W algorithm implemented in MEGA 7.0 (Kumar et al. 2016), and multigene (TUB and TEF) phylogenetic analyses using the neighbor-joining method showed that the isolate was N. aurantiaca. To complete Koch’s postulates, nine 2-week-old peanut (Zhonghua 12) seedlings were sprayed with conidial suspensions (106 conidia ml−1 in 0.05% Tween 20 buffer). The same number of seedlings were treated with 0.05% Tween buffer as controls. The experiment was repeated three times. Plants were incubated in a growth chamber (30°C in the day and 25°C at night, a 12-h photoperiod and 80% RH). Ten days after inoculation, typical symptoms were observed on inoculated leaves but not on the controls. N. aurantiaca was reisolated from the diseased leaves but not from the controls. Nigrospora sphaerica was observed on peanut in China (Liu et al. 2020). To our knowledge, this is the first report of N. aurantiaca causing leaf blight on peanut in Shandong province. These findings will help to develop better preventive measures in accordance with the emergence of the new disease.The author(s) declare no conflict of interest.References:Kumar, S., et al. 2016. Mol. Biol. Evol. 33:1870. https://doi.org/10.1093/molbev/msw054 Crossref, ISI, Google ScholarLiu, X., et al. 2020. Emerg. Microbes Infect. 9:1269. https://doi.org/10.1080/22221751.2020.1773324 Crossref, ISI, Google ScholarWang, M., et al. 2017. Persoonia 39:118. https://doi.org/10.3767/persoonia.2017.39.06 Crossref, ISI, Google ScholarWang, Y., et al. 2022. Plant Dis. 106:323. https://doi.org/10.1094/PDIS-05-21-0900-PDN Link, ISI, Google ScholarFunding: Funding was provided by Natural Science Foundation of Shandong Province (ZR2020QC131, ZR2021QD153); Taishan Scholar Project Funding (tsqn201812121); and Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2021B33, CXGC2022A21).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 107, No. 7 July 2023SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 26 Jul 2023Published: 16 Jun 2023First Look: 5 Dec 2022Accepted: 28 Nov 2022 Page: 2221 Information© 2023 The American Phytopathological SocietyFundingNatural Science Foundation of Shandong ProvinceGrant/Award Number: ZR2020QC131Grant/Award Number: ZR2021QD153Taishan Scholar Project FundingGrant/Award Number: tsqn201812121Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural SciencesGrant/Award Number: CXGC2021B33Grant/Award Number: CXGC2022A21Keywordsleaf blightNigrospora aurantiacapeanutThe author(s) declare no conflict of interest.PDF download