Heat stability of Trichoderma asperelloides SKRU-01 culture filtrates: Potential applications for controlling fungal spoilage and AFB1 production in peanuts
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY(2024)
摘要
This study aimed to examine the heat stability of culture filtrates of Trichoderma asperelloides SKRU-01 (culture filtrates SKRU-01) over a temperatures range (40-121 degrees C) and the effects on the antifungal activity against two aflatoxin-producing strains (Aspergillus parasiticus TISTR 3276 and A. flavus PSRDC-4), aflatoxin B-1 (AFB(1)) degradation, and the role in mycotoxin control in peanuts. The impact of SKRU-01 culture age (2-12 day-old) on both pathogenic strains revealed that the culture age of 6-12 day-old cultures exhibited no significant difference (p > 0.05) of growth inhibition for strain TISTR 3276 (81.89-82.28 %) and 4-12 day-old cultures for strain PSRDC-4 (74.87-79.06 %). The heat-treated temperatures from 40 degrees C to 121 degrees C caused no significant (p > 0.05) reduction of mycelial growth for strain TISTR 3276 (82.61 % to 79.13 %) but significant (p < 0.05) deduction for strain PSRDC-4 (75.15 % to 59.17 %). Heat treatment of the culture filtrates SKRU-01 at 60-121 degrees C caused the reduction on spore germination inhibition (from about 68 % to 58.16 % for strain TISTR 3276 and 51.11 % for strain PSRDC-4). These results indicate that strain TISTR 3276 exhibited greater susceptibility to culture filtrates SKRU-01 compared to strain PSRDC-4. Furthermore, the culture filtrates SKRU-01 exhibited remarkable thermal stability at 121 degrees C, degrading AFB(1) to 63.91 %. Application of heat-stable culture filtrates SKRU-01 in peanuts demonstrated that the reduction in fungal population and AFB(1) production of both pathogenic strains depended significantly (p < 0.05) on the level of heat treatment. The non-treated and 40 degrees C treated culture filtrates SKRU-01 could reduce AFB(1) production to lower than the Standard Aflatoxin Limitation (<20 mu g/kg), ensuring food safety and mitigating the health risks associated with aflatoxin exposure.
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关键词
Culture filtrates,Antifungal activity,AFB(1) degradation,Heat stability,Trichoderma asperelloides SKRU-01
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