The Usability of Extracts of Walnut Green Outer Shell for In vitro Surface Sterilization of Rosehip (Rosa canina L.) Plant

In this study, it was aimed to determine the efficiency ratios of extracts obtained from walnut outer green shell at different concentrations in order to provide surface sterilization in in vitro propagation of rosehip plant. Axillary buds taken in June were used as explants. After surface sterilization of the explants with walnut outer shell extract, they were cultured in MS0 nutrient medium. The lowest contamination rate on the 5th and 7th days was observed in the control and 20K groups. In addition, the highest number of shoots per explant was obtained in the 20K group. Uncontaminated explants were subcultured to MS0 medium containing 1.0 mg /L IBA + 1.0 mg /L BAP and 1.0 mg/L BAP + 1.0 mg/L NAA at the end of the 7th day. Rooting was not observed in the explants due to browning. In the in vitro propagation of plants, extract (20K) obtained from oven-dried walnut shell can be used instead of chemical sterilant.