Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation
bioRxiv (Cold Spring Harbor Laboratory)(2023)
摘要
Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many cellular populations and validation of these populations is highly important. Therefore, in this article, we discuss a comprehensive method to isolate mice skeletal muscle, create satellite cells for tissue culture, and use immunofluorescence to validate our approach.
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关键词
isolated skeletal muscle,skeletal muscle,antibody
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