The prevalence of killer yeasts and double-stranded RNAs in the budding yeast Saccharomyces cerevisiae

Fems Yeast Research(2023)

引用 0|浏览4
暂无评分
摘要
Abstract Killer toxins are antifungal proteins produced by many species of “killer” yeasts, including the brewer's and baker's yeast Saccharomyces cerevisiae. Screening 1 270 strains of S. cerevisiae for killer toxin production found that 50% are killer yeasts, with a higher prevalence of yeasts isolated from human clinical samples and winemaking processes. Since many killer toxins are encoded by satellite dsRNAs associated with mycoviruses, S. cerevisiae strains were also assayed for the presence of dsRNAs. This screen identified that 51% of strains contained dsRNAs from the mycovirus families Totiviridae and Partitiviridae, as well as satellite dsRNAs. Killer toxin production was correlated with the presence of satellite dsRNAs but not mycoviruses. However, in most killer yeasts, whole genome analysis identified the killer toxin gene KHS1 as significantly associated with killer toxin production. Most killer yeasts had unique spectrums of antifungal activities compared to canonical killer toxins, and sequence analysis identified mutations that altered the antifungal activities of killer toxins. The prevalence of mycoviruses and killer toxins in S. cerevisiae is important because of their known impact on yeast fitness, with implications for academic research and industrial application of this yeast species.
更多
查看译文
关键词
killer yeasts,rnas,double-stranded
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要