Holothuria scabra Jaegar 1833 (Sandfish) extracts and collagens modulate protein‐bound N^ε‐carboxymethyllysine, N^ε‐carboxyethyllysine and methylglyoxal‐derived hydroimidazolone‐1 levels

Summary In this study, sea cucumber ( Holothuria scabra ; Jaegar 1833) extracts and collagens were evaluated for inhibitory properties of protein‐bound advanced glycation end products (AGEs). Processed dried sea cucumber with salt extract showed a significant lower IC 50 value for fluorescent AGEs (9.19 ± 7.68 μg mL −1 , P < 0.05) and fructosamine (503.47 ± 46.37 μg mL −1 , P < 0.05), respectively. Processed dried with and without salt extracts significantly reduced the N ε ‐carboxymethyllysine (CML) and methylglyoxal‐derived hydroimidazolone‐1 (MG‐H1) levels tested at 250 μg mL −1 . Smoked dried and fresh‐dried extracts significantly reduced the N ε ‐carboxyethyllysine (CEL) levels tested at 250 μg mL −1 . Pepsin‐solubilised collagen and the crude collagen fibrils significantly reduced CML and MG‐H1 levels whereas CEL levels were unchanged. Pearson's correlation analysis showed that protein‐bound AGE and CML inhibition significantly correlated with the total phenolic and antioxidant activities, respectively. This study provides a strong rationale for further investigation aimed at identifying the active compounds responsible for the observed effects on biomarkers relevant to diabetes.