Effects of Light Treatments on Callus Induction and Morphogenesis in Caladium bicolor

Mengyi Chen, Jiangjiang Xie, Jun‐Lin Guan, Shuangyan Chen, Haiying Huang,Qingwen Luo, Qihua Wang

Research Square (Research Square)(2023)

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Abstract
Caladium bicolor is widely used as an ornamental plant outdoors and indoors due to its rich colors, diverse combinations, and strange patterns. In the commercial production of Caladium bicolor , tissue culture technology can quickly reproduce seedlings with consistent genetic properties. In practice, there are still aspects that can be improved in terms of energy dissipation and ornamental value when using fluorescent lamps. In this experiment, the light intensity had no significant effect on the induction of callus and the germination rate, but from the number of buds per explant increased. Different light waves affect the occurrence of seedlings of Caladium bicolor . Among them, a single light source, LED-Red, is not conducive to callus differentiation, plant height, and carotenoid accumulation compared to other treatments; LED-Blue is beneficial for the formation of relative anthocyanin content and plant height.LED-White is beneficial for leaf size. Fluorescence is not conducive to increasing the total number of seedlings, which is important for production. Compared with fluorescence, the transplanting rate of LED-Blue increased by 94.92%. The results of this experiment suggest that LED light can replace florescent lamps in tissue culture for achieving low energy consumption and high efficiency.
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