Chiral quantitative analysis of Loperamide in a state of acid degradation by HPLC method

Abstract In this work the stability of Loperamide® hydrochloride has been described in different pH solutions with different buffer species. High performance liquid chromatography (HPLC) is an efficiency method of Loperamide® hydrochloride, but the direct analysis procedure is not suitable for the determination of Loperamide® hydrochloride in the presence of its acid degradation product or its impurity. The present work aims to develop and validate a new feasible, sensitive and specific analytical procedure using HPLC on chiral columns.