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Functional characterization, structural basis, and regio-selectivity control of a promiscuous flavonoid 7,4′-di-O-glycosyltransferase from Ziziphus jujuba var. spinosa

Zilong Wang,Wanqing Wei,Haidong Wang,Jia-Jing Zhou,Haotian Wang,Kuan Chen,Rong-Shen Wang,Fudong Li,Xue Qiao, Huan‐Xiang Zhou,Yong Liang,Min Ye

Chemical Science（2023）

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Abstract
We report a promiscuous flavonoid 7,4′-di- O -glycosyltransferase (ZjOGT3), which shows high catalytic efficiency and regio-selectivity. And the regio-selectivity of ZjOGT3 was controlled by intrinsic reactivity and the enzyme microenvironment.
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Key words
promiscuous flavonoid,regio-selectivity
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