Protection of LP-cs on acute alcohol-induced liver and intestine injury

Abstract The beneficial effects of probiotics have been studied extensively in inflammatory bowel disease, nonalcoholic steatohepatitis (NASH), and alcoholic liver disease (ALD). Probiotic supplements are considered safer and more effective, but the potential mechanisms behind their benefits are unclear. An objective of the current study was to examine the effects of extracellular products of Lactobacillus plantarum on acute alcoholic liver injury. Mice on standard chow diet were supplemented with Lactobacillus plantarum ST-III culture supernatant (LP-cs) for 2 weeks and administered a dose of alcohol at 6 g/kg body weight by gavage. Alcohol-induced liver injury was assessed by measuring plasma alanine aminotransferase (ALT) activity levels, and liver steatosis was determined by triglyceride content. Intestine was measured by H&E staining and tight junction proteins were examined. LP-cs significantly inhibited the alcohol-induced fat accumulation, inflammatory reaction, and apoptosis by inhibiting oxidative stress and ER stress. In addition, LP-cs significantly inhibited the alcohol-induced intestinal injury and endotoxemia. According to these findings, LP-cs alleviates the acute alcohol-induced liver damage by inhibiting oxidative stress and ER stress in one way and suppressing alcohol-induced increased intestinal permeability and endotoxemia in another way. Our findings indicated that LP-cs supplements provided a novel strategy for ALD preventions and treatments.