Production of Alginate Biofilm Added With Vanillin for Application as a Biocurative

Abstract The present work presents the results of the production of vanillin-doped alginate biofilm. Alginate dressings are already a therapeutic reality, but they act only by maintaining the appropriate environment for healing. With the intention of improving their properties, the incorporation of vanillin was proposed due to its antioxidant and antimicrobial potential. Different biofilms were produced employing experiment planning through response surface analysis, which allowed determining the best region for a medium value of solubility and high degree of intumescence. This region refers to values above 0.07 g of CaCl 2 and concentrations above 0.024 g of vanillin, triggering a solubility between 25% and 30% and a degree of intumescence above 100% and with fixed values of alginate (0.85 g). As for the optimization of the process, the Normal Boundary Intersection (NBI) method was able to analyze concave regions, predicting the optimal points and generating the Pareto chart with equidistant limits The antimicrobial test allowed the observation of antimicrobial activity against Escherichia coli and Pseudomonas aeruginosa microorganisms from the biofilms, as well as a solution of vanillin with calcium chloride and glycerol obtaining a halo of inhibition only in the presence of vanillin. Thermal analysis of thermogravimetry and differential exploratory calorimetry showed that the material has thermal stability, in the temperature range ideal for application as a biomaterial, for example as biocuratives.