In vivoStability and Biodistribution of Liposome Coated with SlpB fromLevilactobacillus brevis

Abstract SlpB from Levilactobacillus brevis offers a solution to stabilise liposome in gastrointestinal tract, and to target intestinal APCs in Peyer’s patches, rendering it a powerful tool for oral delivery of drugs, and to yield the benefits provided by oral delivery. However, the stability of SlpB-coated liposome (SlpB-LP) and its distribution in tissues were not characterized. In this study, we have demonstrated that SlpB-coating could improve the stability of liposome in gastrointestinal tract, and facilitate specific uptake of liposome into Peyer’s patches, but not intestinal, nor intestinal mucosa. Furthermore, we have shown that uptake of SlpB-LP into Peyer’s patches enhanced bioavailability of drugs, which have resulted in 427.65-fold increase in bioavailability and at least 2.41-fold decrease in retention of fluorophore in liver where drug metabolism takes places, to a degree which approximate control group. In conclusion, this study shows that SlpB could increase stability of liposome in gastrointestinal tract, increase specific uptake of liposome into Peyer’s patches, and improve bioavailability. Abstract Figure