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Superior induced pluripotent stem cell generation through Phactr-3-driven mechano-modulation of both early and late phases of cell reprogramming

Mohammad Mahfuz Chowdhury,Samuel Zimmerman,Hannah Leeson,Christian Maximilian Nefzger,Jessica Cara Mar,Andrew Laslett,Jose Maria Polo,Ernst Wolvetang,Justin Cooper-White

Biomaterials Research（2024）

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Abstract
Abstract Background: Human cell reprogramming traditionally involves time-intensive, multi-stage, costly tissue culture polystyrene (TCPS)-based cell culture practices that ultimately produce low numbers of reprogrammed cells of variable quality. Previous studies have shown that very soft two-and three-dimensional hydrogel substrates/matrices (of stiffnesses More
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Key words
pluripotent stem cell generation,mechano-modulation
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