Isolation, identification and antibiotic susceptibility of gram-negative anaerobic bacteria in oral cavity

Gram-negative bacteria include more than 20 genera. The most commonly isolated genera are: Bacteriodes spp., Porphiromonas spp., Fusobacterium spp. and Prevotella spp. The following genera were isolated somewhat less frequently: Tanerella spp., Leptotricha spp., Veilonella spp.,Wollinela spp., Selenomonas spp. and Treponema spp. Anaerobic bacteria have an anaerobic type of metabolism and therefore their incubation is significantly longer and more demanding than aerobic bacteria. The genera Prevotella spp., Porphyromonas spp. and Fusobacterium spp. are part of the resident flora of the oral cavity and in unfavorable conditions cause periodontal diseases, and sometimes dentogenic infections and systemic diseases, such as Alzheimer?s disease, cardiovascular diseases, metabolic diseases and inflammatory bowel diseases. The three most important steps for the successful diagnosis of anaerobic bacteria are: proper sampling with avoiding sample contamination, rapid transport of samples to the microbiological laboratory and proper handling of samples. A combination of beta-lactam with the addition of beta-lactamase inhibitors, metronidazole, clindamycin and moxifloxacin is used for treatment for infections caused by anaerobic Gram-negative bacteria. It is important to note that antibiotics should be used only with a clear indication and to choose the right antibiotic in the optimal dose. The aim of this review is to point out the role of Gram-negative anaerobic bacteria in periodontal diseases, and its isolation, identification and antibiotic susceptibility.