Blood+ proteomics reveals hemorrhagic shock mechanisms in a severe hemorrhagic shock rat model

ABATRACT: Hemorrhagic shock is one of the most important complications of war trauma and an important cause of early death from trauma, so timely diagnosis and treatment are of overwhelming importance. A rat model of 65% severe hemorrhagic shock was established to analyze the different proteins in the plasma between normal rats and predict the mechanism preliminarily by Blood+ proteomics. The results showed that a total of 190 regulated proteins were detected in plasma in rats with severe hemorrhagic shock compared to normal rats. Bioinformatics and enrichment analysis of regulated proteins showed that they were widely distributed in 45 signaling pathways. Of these, 25 of the 190 proteins were enriched in the PI3K-Akt signaling pathway, indicating a strong link between this pathway and severe ischemia. Other enrichment pathways in this study include primary immunodeficiency (PID), toll-like receptor signaling pathway, ECM-receptor interaction, all of which are closely related to autoimmune disorders, inflammatory responses, and anti-inflammatory responses. In conclusion, this study revealed an overview of the framework of protein changes in the plasma of rats with severe hemorrhagic shock, and preliminarily analyzed the mechanism of initial response to severe ischemia.