Analysis of ATH1 Transcription Activity and Co-IP of ATH1 Protein Interaction with OFP1

ATH1 has multifaceted functions in regulating growth and development, and possibly regulate development by interacting with OFP1 protein. However, the protein interaction of ATH1 with OFP1 has not been further confirmed, and the transcription activity of ATH1 has not been determined. In this work, the interaction ATH1 with OFP1 was further confirmed by using Co-IP, and the transcription activity of ATH1 was determined by using Protoplast transfection system. The results showed that the ATH1-OFP1 protein complexes could be formed in vivo , and ATH1 was a transcription inhibitory factor, and OFP1 could enhance the transcription inhibitory activity of ATH1. This study will provide molecular basis for analysis of the regulatory functions of OFP-TALE complexes in plant development, which is great significance for improving important plant traits.